بررسی مشخصات مکانیکی و دوام بتن خود ترمیم شونده حاوی باکتری رسوب دهنده کربنات کلسیم ایموبلایز شده در پرلیت

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه میکروبیولوژی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران

2 گروه مهندسی عمران، واحد تهران غرب، دانشگاه آزاد اسلامی، تهران، ایران

3 گروه میکروبیولوژی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

10.22124/jcr.2022.21339.1544

چکیده

امروزه ترکیبات خود ترمیم کننده بعنوان مصالح هوشمند در بهبود خصوصیات مواد، در بسیاری از صنایع مورد استفاده قرار می‌گیرند. یکی از روشهای خود ترمیمی بتن، استفاده از مکانیسم رسوب کربنات کلسیم ناشی از فعالیت میکروبی می‌باشد. این مقاله به بررسی تأثیر استفاده از باکتری اسپورو‌سارسینا در بهبود مشخصات مکانیکی و دوام بتن پرداخته است. به این منظور از سنگدانه پرلیت بعنوان حامل استفاده شده و باکتری در آن ایموبلایز شده است.

باکتری اسپورو‌سارسینا در پنج غلظت مختلف (cfu/ml 109×7/6 و 109×2/5 ، 109×4 ، 109×6/2 ،109×5/1) در بتن مورد استفاده قرار گرفته و با آزمونه شاهد فاقد باکتری مقایسه شده است. بعد از ساخت و عمل آوری، پارامترهای مختلف از قبیل مقاومت فشاری، مقاومت کششی، میزان نفوذپذیری در برابر آب و یون کلر در آزمونه‌ها در سه سن 7، 28 و 90 روزه مورد ارزیابی قرار گرفت. همچنین ریزساختار تعدادی از مخلوط‌ها با استفاده از میکروسکوپ الکترونی روبشی بررسی شد. نتایج آزمایش‌های انجام شده نشان داد که مقاومت فشاری و مقاومت کششی آزمونه‌های حاوی باکتری به علت فعالیت میکروبی با افزایش روبرو شده و میزان نفوذپذیری آن کاهش می‌یابد. میزان افزایش مقاومت فشاری در نمونه حاوی باکتری با غلظت cfu/ml 109×6/2 حدود بیست و سه درصد اندازه‌گیری گردید.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Mechanical and durability properties of self-healing concrete containing carbonate precipitation bacteria immobilized in perlite

نویسندگان [English]

  • Motahhareh Nezafat Tabalvandani 1
  • Mehdi Esfandi Sarafraz 2
  • Maryam Tajabadi-Ebrahimi 1
  • Abbas Akhavan Sepahy 3
1 Department of Microbiology, Islamic Azad University, Central Tehran Branch, Tehran, Iran
2 Department of Civil Engineering, Islamic Azad University, West Tehran Branch, Tehran, Iran
3 Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran
چکیده [English]

Today, self-healing materials are used as intelligent materials in improving the properties of structures in many industries. One of the methods of self-healing in concrete is the use of calcium carbonate precipitation mechanism due to microbial activities. This research evaluated the effects of a specific strain of Sporosarcina pasteurii bacteria as a new method on the mechanical and durability properties of self-healing concrete. For this purpose, perlite aggregate was used as a carrier, and bacteria were immobilized in it.

Five different cell concentrations (1.5×109, 2.6×109, 4×109, 5.2×109, 6.7×109 cfu /ml) of bacteria were used in the concrete mixtures and compared with a control mixture without bacteria. The compressive strength, tensile strength, water permeability, and chloride ion penetration at the age of 7, 28, and 90 days were tested. Also, the microstructure of some mixtures was investigated by Scanning Electron Microscope. The test results demonstrated that the use of Sporosarcina pasteurii immobilized in perlite improved the compressive and tensile strength, reduced the permeability of concrete. Maximum increase (23%) in compressive strength was measured with 2.6×109 cfu/ml of bacteria.

کلیدواژه‌ها [English]

  • Self-healing concrete
  • Bacteria
  • Calcium carbonate precipitation
  • Compressive strength
  • Durability
[1]       D. G. Bekas, K. Tsirka, D. Baltzis, and A. S. Paipetis, “Self-healing materials: A review of advances in materials, evaluation, characterization and monitoring techniques,” Composites Part B: Engineering, vol. 87, pp. 92–119, 2016.
[2]       W. De Muynck, N. De Belie, and W. Verstraete, “Microbial carbonate precipitation in construction materials: A review,” Ecological Engineering, vol. 36, no. 2, pp. 118–136, 2010.
[3]       W. De Muynck, K. Cox, N. De Belie, and W. Verstraete, “Bacterial carbonate precipitation as an alternative surface treatment for concrete,” Construction and Building Materials, vol. 22, no. 5, pp. 875–885, 2008.
[4]       W. De Muynck, D. Debrouwer, N. De Belie, and W. Verstraete, “Bacterial carbonate precipitation improves the durability of cementitious materials,” Cement and Concrete Research, vol. 38, no. 7, pp. 1005–1014, 2008.
[5]       V. Wiktor and H. M. Jonkers, “Quantification of crack-healing in novel bacteria-based self-healing concrete,” Cement and Concrete Composites, vol. 33, no. 7, pp. 763–770, 2011.
[6]       V. Achal, X. Pan, and N. Özyurt, “Improved strength and durability of fly ash-amended concrete by microbial calcite precipitation,” Ecological Engineering, vol. 37, no. 4, pp. 554–559, 2011.
[7]       F. Pacheco-Torgal and J. A. Labrincha, “Biotech cementitious materials: Some aspects of an innovative approach for concrete with enhanced durability,” Construction and Building Materials, vol. 40, pp. 1136–1141, 2013.
[8]       R. Siddique and N. K. Chahal, “Effect of ureolytic bacteria on concrete properties,” Construction and Building Materials, vol. 25, no. 10, pp. 3791–3801, 2011.
[9]       S. S. Bang, J. K. Galinat, and V. Ramakrishnan, “Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii,” Enzyme and Microbial Technology, vol. 28, no. 4, pp. 404–409, 2001.
[10]     N. Chahal, R. Siddique, and A. Rajor, “Influence of bacteria on the compressive strength, water absorption and rapid chloride permeability of fly ash concrete,” Construction and Building Materials, vol. 28, no. 1, pp. 351–356, 2012.
[11]     R. Siddique, K. Singh, Kunal, M. Singh, V. Corinaldesi, and A. Rajor, “Properties of bacterial rice husk ash concrete,” Construction and Building Materials, vol. 121, pp. 112–119, 2016.
[12]     R. Vashisht, S. Attri, D. Sharma, A. Shukla, and G. Goel, “Monitoring biocalcification potential of Lysinibacillus sp. isolated from alluvial soils for improved compressive strength of concrete,” Microbiological Research, vol. 207, pp. 226–231, 2018.
[13]     M. Seifan, A. K. Sarmah, A. K. Samani, A. Ebrahiminezhad, Y. Ghasemi, and A. Berenjian, “Mechanical properties of bio self-healing concrete containing immobilized bacteria with iron oxide nanoparticles,” Applied Microbiology and Biotechnology, vol. 102, no. 10, pp. 4489–4498, 2018.
[14]     M. Seifan, A. Ebrahiminezhad, Y. Ghasemi, A. K. Samani, and A. Berenjian, “Amine-modified magnetic iron oxide nanoparticle as a promising carrier for application in bio self-healing concrete,” Applied Microbiology and Biotechnology, vol. 102, no. 1, pp. 175–184, 2018.
[15]     N. Parastegari, D. Mostofinejad, and D. Poursina, “Use of bacteria to improve electrical resistivity and chloride penetration of air-entrained concrete,” Construction and Building Materials, vol. 210, pp. 588–595, 2019.
[16]     M. Pourfallahi, A. Nohegoo-Shahvari, and M. Salimizadeh, “Effect of direct addition of two different bacteria in concrete as self-healing agent,” Structures, vol. 28, pp. 2646–2660, 2020.
[17]     BS 12390-3:2009, “12390-3 (2009) Testing hardened concrete—part 3: compressive strength of test specimens,” British Standards Institution, 2009.
[18]     ASTM C496M-12, “Standard Test Method for Splitting Tensile Strength of Cylindrical Concrete Specimens,” ASTM International, West Conshohocken, PA, 2012.
[19]     BS 12390-8:2009, “12390-8 (2009) Testing hardened concrete—part 8: Depth of penetration of water under pressure,” British Standards Institution, 2009.
[20]     ASTM C1202-12, “Standard Test Method for Electrical Indication of Concrete’s Ability to Resist Chloride Ion Penetration,” ASTM International, West Conshohocken, PA, 2012.
[21]     V. Achal, A. Mukerjee, and M. Sudhakara Reddy, “Biogenic treatment improves the durability and remediates the cracks of concrete structures,” Construction and Building Materials, vol. 48, pp. 1–5, 2013.
[22]     W. Khaliq and M. B. Ehsan, “Crack healing in concrete using various bio influenced self-healing techniques,” Construction and Building Materials, vol. 102, pp. 349–357, 2016.
[23]     P. Ghosh, S. Mandal, B. D. Chattopadhyay, and S. Pal, “Use of microorganism to improve the strength of cement mortar,” Cement and Concrete Research, vol. 35, no. 10, pp. 1980–1983, 2005.
[24]     R. Andalib et al., “Optimum concentration of Bacillus megaterium for strengthening structural concrete,” Construction and Building Materials, vol. 118, pp. 180–193, 2016.
[25]     C. Sonali Sri Durga, N. Ruben, M. Sri Rama Chand, and C. Venkatesh, “Performance studies on rate of self healing in bio concrete,” Materials Today: Proceedings, vol. 27, pp. 158–162, 2020.
[26]     V. Achal, A. Mukherjee, and M. S. Reddy, “Effect of calcifying bacteria on permeation properties of concrete structures,” Journal of Industrial Microbiology and Biotechnology, vol. 38, no. 9, pp. 1229–1234, Sep. 2011.