Effect of Sporosarcina bacteria and fly ash on concrete self-healing ability

Document Type : Research Paper

Authors

1 2- Department of Civil Engineering, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.

2 2- Department of Civil Engineering, Meymand center, Islamic Azad University, Meymand, Iran.

10.22124/jcr.2022.21953.1568

Abstract

In this paper, the effect of bacteria on self-healing concrete with fly ash has been studied. Sporosarcina bacteria with four different concentrations were used for evaluation. In concrete with fly ash, fly ash is replaced by 20% by weight of cement of mixing design has been used. In order to evaluate and control the specimen, destructive and non-destructive tests such as ultrasonic, permeability, water absorption and compressive strength have been used. To evaluate the effect of bacteria on self-healing of concrete cracks, concrete cracks were studied using scanning electron microscopy (SEM) and light microscope. The results show that the concentration of 10.46 × 106 is the most suitable concentration of Sporosarcina for the production of calcite and filling the pores of concrete by products such as calcium carbonate. The mentioned concentration resulted in a 36% increase in compressive strength in specimen with fly ash and a 30% increase in specimen without fly ash. The results also showed that increasing the bacterial concentration did not cause significant changes in compressive strength, water absorption, impermeability and ultrasonic wave velocity.

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Main Subjects

References

[1] Li. W, Lin. X, Bao. D. W, Xie. Y. M, A review of formwork systems for modern concrete construction,Structures,Volume 38: 52-63, 2022.
[2] Mohan. M, Rahul. A. V,van Dam. B, Zeidan. T,Schutter. G. D, Tittelboom. K. V, Performance criteria, environmental impact and cost assessment for 3D printable concrete mixtures,Resources, Conservation and Recycling,Volume 181:106255, 2022.
[3] Aspiotis. K, Sotiriadis. K, Ntaska. A, Macova. P, Badogiannis. E, Tsivilis. S,durability assessment of self-healing in ordinary Portland cement concrete containing chemical additives, Construction and Building Materials, Volume 305:124754, 2021.
[4] Hassanpour. M, Hassanpour. M, Rezaie. M, Salajegheh. E, Iqbal. M. R, Khandaker. M. U, Bradley. D. A, Studies of the mechanical and neutron shielding features of concrete by incorporation of green additive materials: Experimental and numerical study, Radiation Physics and Chemistry, Volume 191:109846, 2022.
[5] Mokhtar. M. M, Morsy. M,Taha. N. A, Ahmed. E. M, Investigating the mechanical performance of nano additives reinforced high-performance concrete, Construction and Building Materials, Volume 320:125537, 2022.
[6] Shashank. B. S, Kumar. K. P, Nagaraja. P. S, Fracture behavior study of self-healing bacterial concrete, Materials Today: Proceedings, 2022.
[7] Khaudiyal. S, Rawat. A, Kumar Das. S, Garg. N, Bacterial concrete: A review on self-healing properties in the light of sustainability, Materials Today: Proceedings, 2021.
[8] Tang. Y, Xu. J, Application of microbial precipitation in self-healing concrete: A review on the protection strategies for bacteria,
Construction and Building Materials, Volume 306:124950, 2021.
[9] Reddy. K. M, Ramesh. B, Macrin. D, Effect of crystalline admixtures, polymers and fibers on self-healing concrete - a review, Materials Today: Proceedings, Volume 33:763-770, 2020.
[10] Jogi. K. P, Lakshmi. T. V. S, Self-healing concrete based on different bacteria: A review, Materials Today: Proceedings, Volume 43:1246-1252, 2021.
[11] Reddy. Y. P. V, Ramesh. B, Prem Kumar. L, Influence of bacteria in self-healing of concrete - a review, Materials Today: Proceedings,
Volume 33:4212-4218, 2020.
[12] Zhang. W, Zheng. Q, Ashour. A, Han. B, Self-healing cement concrete composites for resilient infrastructures: A review, Composites Part B: Engineering, Volume:189:107892, 2020.
[13] Zhang. L. V, Nehdi. M. L, Suleiman. A. R, Mehdizadeh Allaf. M, Gan. M, Marani. A,Tuyan. M, Crack self-healing in bio-green concrete, Composites, Engineering, Volume 227:109397, 2021.
[14] Algaifi. H. A, Abu Bakar. S, Alyousef. R, Mohd Sam. A. R, Wan Ibrahim. M. H, Shahidan. S,Ibrahim. M, Salami. B. A, Bio-inspired self-healing of concrete cracks using new B. pseudomycoides species,Journal of Materials Research and Technology, Volume 12:967-981, 2021.
[15] Peng. C, Wu. Q, Shen. J, Mo. R, Xu. J, Numerical study on the effect of transverse crack self-healing on the corrosion rate of steel bar in concrete, Journal of Building Engineering, Volume 41:102767, 2021.
[16] Sri Durga. C. S, Ruben. N, Rama Chand. M. S, Indira. M, Venkatesh. C, Comprehensive microbiological studies on screening bacteria for self-healing concrete, Materialia, Volume 15:101051, 2021.
[17] Sohail. M. G, Disi. Z. A, Zouari. N, Al Nuaimi. N, Kahraman. R, Gencturk. B, Rodrigues. D. F, Yildirim. Y, Bio self-healing concrete using MICP by an indigenous Bacillus cereus strain isolated from Qatari soil, Construction and Building Materials, Volume 328:126943, 2022.
[18] Feng. F, Chen. B, Sun. W, Wang. Y, Microbial induced calcium carbonate precipitation study using Bacillus subtilis with application to self-healing concrete preparation and characterization, Construction and Building Materials, Volume 280:122460, 2021.
[19] Su. Y, Zheng. T, Qian. C, Application potential of Bacillus megaterium encapsulated by low alkaline sulphoaluminate cement in self-healing concrete, Construction and Building Materials, Volume 273:121740, 2021.
[20] Nguyen. T, Ghorbel. E, Fares. H, Cousture, A, Bacterial self-healing of concrete and durability assessment, Cement and Concrete Composites, Volume 104:103340, 2019.
[21] Chen. B, Sun. W, Sun. X, Cui. C, Lai. J, Wang. Y, Feng. J, Crack sealing evaluation of self-healing mortar with Sporosarcina pasteurii: Influence of bacterial concentration and air-entraining agent,
Process Biochemistry, Volume 107:100-111, 2021.
[22] Hosseini Balam. N, Mostofinejad. D, Eftekhar. M, Effects of bacterial remediation on compressive strength, water absorption, and chloride permeability of lightweight aggregate concrete, Construction and Building Materials, Volume 145:107-116, 2017.
[23] Omoregie. A, Palombo. E, Ong. D, Nissom. P, A feasible scale-up production of Sporosarcina pasteurii using custom-built stirred tank reactor for in-situ soil biocementation, Biocatalysis and Agricultural Biotechnology, Volume 24:101544, 2020.
[24] Xiangliang Pan. V, Daoyong Zhang. O,
Biomineralization based remediation of as (III) contaminated soil by Sporosarcina ginsengisoli, Journal of Hazardous Materials, Volumes 201:178-184, 2012.
[25] Fu. Q, Wu. Y, Liu. S, Lu. L, Wang. J, The adaptability of Sporosarcina pasteurii in marine environments and the feasibility of its application in mortar crack repair, Construction and Building Materials, Volume 332: 127371, 2022.
[26] Mirshahmohammad. M, Rahmani. H, Maleki-Kakelar. M, Bahari. A, Effect of sustained service loads on the self-healing and corrosion of bacterial concretes, Construction and Building Materials, Volume 322:126423, 2022.
[27] Cuzman. O, Rescic. S, Richter. K, Wittig. L, Tiano. P, Sporosarcina pasteurii use in extreme alkaline conditions for recycling solid industrial wastes, Journal of Biotechnology, Volume 214:49-56, 2015.
[28] Hadi. S, Abbas. H, Almajed. A, Binyahya. A, Al-Salloum. Y, Biocementation by Sporosarcina pasteurii ATCC6453 under simulated conditions in sand columns, Journal of Materials Research and Technology, 2022.
[29] Feng. J, Dong. H, Wang. R, Su. Y. A, novel capsule by poly (ethylene glycol) granulation for self-healing concrete, Cement and Concrete Research, Volume 133:106053, 2020.
[30] Lv. L, Guo. P, Liu. G, Han. N, Xing. F, Light induced self-healing in concrete using novel cementitious capsules containing UV curable adhesive, Cement and Concrete Composites, Volume 105:103445, 2020.
[31] Pourfallahi. M, Nohegoo-Shahvari. A, Salimizadeh. M, Effect of direct addition of two different bacteria in concrete as self-healing agent, Structures, Volume 28:2646-2660, 2020.
[32] Nasim. N, Dewangan. U. K., S. V. Deo, Effect of crystalline admixture, fly ash, and PVA fiber on self-healing capacity of concrete, Materials Today: Proceedings, Volume 32:844-849, 2020.
[33] Durga. P, Singh. R. N, Climatic, pathogenic and host conditions for successful induction of Ustilaginoidea virens causing false smut of rice. Indian Journal of Ecology, Volume 44(5):407-411. 2017.
[34] Reddy. N. R, Tetzloff. R. C, Solomon. H. M, Larkin. J. W, Inactivation of Clostridium botulinum nonproteolytic type B spores by high pressure processing at moderate to elevated high temperatures, Innovative Food Science & Emerging Technologies, Volume 7(3):169-175,2006.
[35] ACI committee 211, ACI 211. 1-91, standard practice for selecting proportions for normal, heavy weight, and mass concrete, formington Hills, MI, USA, 2002.
[36] ASTM C192/C192M-16, Standard Practice for Making and Curing Concrete Test Specimens in the Laboratory.
[37] ASTM C 597 – 02 Standard Test Method for Pulse Velocity Through Concrete.
[38] ASTM C618, standard test method for Compressive strength of cylindrical compressive specimen, ASTM international, west Conshohocken,
PA.
[39] ASTM C642, standard test method for density, absorption, and voids in hardened concrete, ASTM international, west Conshohocken, PA. 2006
[40] BS EN 12390, Testing hardened concrete Part 122, Method for determination of water absorption.
[41] ISI 7031," concrete hardened-determination of depth of penetration of water under pressure", ed, 1998.
[42] BS EN 12390-8: 2009, Depth of penetration of water under pressure.

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